Recombinant Dna Vaccines by Richard Isaacson

By Richard Isaacson

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Ginsberg, F. Brown, R. A. Lerner, and R. M. ). Cold Spring Harbor Laboratory, New York, pp. 8. Schoolnik, G. K. (1982). Mechanism of binding of gonococcal pili. In: Microbiology 1982 (D. ). American Society of Microbiology, Washington, DC, pp. 312316. 9. , and Mowat, N. (1986). Protection of cattle against food and mouth disease virus by a synthetic peptide. Science 232:639641. 10. Sherman, D. , Acres, S. , Sadowski, P. , Springer, J. , Raybould, T. J. , and Muscoplat, C. C. (1983). Protection of calves against fatal enteric colibacillosis by orally administered Escherichia coli K99-specific monoclonal antibody.

372--dc20 92-17708 CIP This book is printed on acid-free paper. Copyright © 1992 by Marcel Dekker, Inc. All rights reserved. Neither this book nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, microfilming, and recording, or by any information storage and retrieval system, without permission in writing from the publisher. Marcel Dekker, Inc. 270 Madison Avenue, New York, New York 10016 Current printing (last digit): 10 9 8 7 6 5 4 3 2 1 PRINTED IN THE UNITED STATES OF AMERICA Page iii PREFACE Jenner's discovery of the principle of vaccination in 1796 was a major step toward the reduction of fatal, frequently epidemic infections.

During only one phase is the 987P pilus-adhesin produced. Since this is an all or none process, it is essential to control the cultivation of this organism when producing the E. coli vaccine described previously. Like B. pertussis, expression of the piliated phase for 987P pilus-adhesin is not favored in vitro. Some Additional Comments Two additional technologies deserve discussion: synthetic peptide vaccines and monoclonal antibody-based vaccines. The advent of rapid DNA sequencing techniques have greatly simplified the ability to determine the primary sequence of proteins.

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