Organic Vegetable Gardening (PB1391)

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The polymerization is carried out in a sealed container. After completion of the polymerization, the container is broken and the polymer monolith is coarsely crushed, then ground and sieved to obtain particles of appropriate size range. The template molecules are extracted from the polymer. Finally, the polymer particles are dried. This protocol is time- and labor-intensive and produces large amounts of particles of unwanted size fractions. 5). 5 Scanning electron micrograph of irregular MIP particles.

The rebinding, however, relies on noncovalent interactions. When the covalently bound template moiety is cleaved from the MIP, a functional group capable of interacting non-covalently with the target molecule is left behind in the polymer at the cleavage site. The semi-covalent approach has been used successfully for the preparation of MIPs selective for p-aminophenylalanine ethyl ester [69], phenol [70] and triazines [71] among others. The cavity left behind after cleaving off the covalently bound template moiety is often too small to accommodate the target molecule during non-covalent rebinding.

Meldal, M. and Bock, K. (1999) Journal of the American Chemical Society, 121, 5459–66. 44 Atherton, E. C. (1989) Solid Phase Peptide Synthesis: A Practical Approach, IRL Press, Oxford. 45 Kempe, M. and Barany, G. (1996) Journal of the American Chemical Society, 118, 7083–93. 46 Meldal, M. (1992) Tetrahedron Letters, 33, 3077–80. 47 Renil, M. and Meldal, M. (1996) Tetrahedron Letters, 37, 6185–8. 48 Buchardt, J. and Meldal, M. (1998) Tetrahedron Letters, 38, 8695–8. , Meldal, M. and Bock, K. (1999) Chemistry – A European Journal, 5, 1088–95.

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