Mobile Health Technologies: Methods and Protocols by Avraham Rasooly, Keith E. Herold

By Avraham Rasooly, Keith E. Herold

This quantity represents a worthwhile choice of cellular healthiness (mHealth) rising applied sciences. Chapters specialise in 3 major components of mHealth: applied sciences for in vitro and environmental checking out, mHealth applied sciences for physiological and anatomical measurements and mHealth applied sciences for imaging. This booklet is designed to make mHealth extra obtainable and comprehensible to engineers, doctors, molecular biologists, chemical, and actual technological know-how researchers constructing mHealth applied sciences.

Written within the hugely profitable Methods in Molecular Biology sequence layout, chapters contain introductions to their respective issues, technical information regarding fabrics and reagents, step by step, with ease reproducible laboratory protocols, and key pointers on troubleshooting and averting identified pitfalls.

Authoritative and sensible, Mobile HealthTechnologies : tools in Molecular Biology goals to enhance entry to clinical tactics together with early detection, diagnostics and remedy in the course of the improvement of recent transportable and obtainable units, and that it will result in stronger healthiness technologies.

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Wolfe KA, Breadmore MC, Ferrance JP, Power ME, Conroy JF, Norris PM, Landers JP (2002) Toward a microchip-based solid-phase extraction method for isolation of nucleic acids. Electrophoresis 23:727–733 77. Chung YC, Jan M-S, Lin Y-C, Lin J-H, Cheng W-C, Fan C-Y (2004) Microfluidic chip for high efficiency DNA extraction. Lab Chip 4:141–147 78. Gudnason H, Dufva M, Bang DD, Wolff A (2007) Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature.

Traditionally, plasma is separated from blood by centrifugation. Centrifugation is, however, inappropriate for on-site applications. As an alternative means, we developed a passive, lowcost, pump-free separator (Fig. 4) that utilizes both sedimentation and filtration to extract plasma from whole blood [89]. , Port Washington, NY). Red cells, white cells, and platelets are trapped without lysis in the large pores on the upstream side of the membrane. The plasma outflows through the smaller pores on the membrane’s downstream side.

Also, too low a temperature will reduce amplification efficiency. 5. Limit of detection in the chip is significantly lower than parallel benchtop assay. Possible causes: (a) Non-optimal temperature control. Check amplification reactor temperature with a calibration chip equipped with temperature sensor. (b) Fluorescent dyes degraded. Dyes lose their fluorescence over time due to bleaching and excessive freeze-thaw. Minimize exposure of dyes to light as much as possible. Dyes can be mixed with calibration DNA (such as gel marker ladders) and tested in chip with fluorometer.

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