High-Performance Thin-Layer Chromatography (HPTLC) by MM. Srivastava (auth.), ManMohan Srivastava (eds.)
By MM. Srivastava (auth.), ManMohan Srivastava (eds.)
The ebook is prepared in 4 chapters comprising of 18 contributions from specialists of foreign reputation. The publication highlights HPTLC procedure as a flexible sleek analytical approach near to its first-class automation, optimization, multidimensional functions. It bargains with basics, precept, conception of HPTLC established separation, experimental information, dealing with knowing and implementation. It describes a number of purposes of HPTLC separation with exact connection with phytochemical research, fingerprint research, authentication, biomedical research, drug quantification, analytical research, quantitative research and validation. HPTLC and its destiny in combinatorial strategy provides new size for its power for hyphenation, HPTLC-MS coupling, laser desorption-HPTLC chromatography coupled with chemical ionization mass spectrometry etc.
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Extra resources for High-Performance Thin-Layer Chromatography (HPTLC)
Selectivity Selectivity of separation is seldom referred to in the case of thin-layer chromatography, although no serious reason can be given for avoiding this term. S. Variyar et al. which particular technique, column or planar, is being considered. In the case of thin-layer chromatography, the separation factor a can be defined as a ¼ K2/K1 (14) which remains in full conformity with the definition used for the column technique. 13) describing the resolution Rs of two overlapping chromatographic spots.
In this case, the Rf values become systematically too large. The loss of mobile phase or the “piling up” of mobile-phase components already present results in the values becoming too small. These Rf-values are not real and cannot be used to calculate k, the capacity factor. Real values can be obtained when no gradient exists along the separation path, no loss of mobile phase occurs, the correct position of the solvent front can be measured without errors, and by excluding any influences resulting from prevaporization.
Dr. Luftmann Head of the Mass Spectrometry Department at the Institute of Organic Chemistry of the University of Munster, Germany, developed an interface (ChromeXtractor) which allows such HPTLC–MS hyphenation. Dr. Morlock, assistant professor at the Institute of Food Chemistry of the University of Hohelnheim in Stuttgart, Germany, modified ChromeXtractor and demonstrated the performance of this versatile interface in comparison to other technical solutions for hyphenation. The substance of interest is eluted directly form the HPTLC plate and is transferred online into the mass spectrometer.