Fibrosis Research: Methods and Protocols by John Varga, David A. Brenner, Sem H. Phan

By John Varga, David A. Brenner, Sem H. Phan

Prime investigators evaluation the highlights of present fibrosis study and the experimental methodologies used discover the mechanisms that force it. of their dialogue of study methodologies using cultured cells to version a variety of features of the fibrotic reaction in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and spotlight the similarities and ameliorations among tools which are acceptable for various varieties of fibroblasts. techniques for learning collagen gene rules and TGF-b construction also are mentioned, in addition to experimental methodologies using animal versions to review the pathogenesis of fibrosis. The protocols keep on with the winning tools in Molecular medication™ sequence structure, each one providing step by step laboratory directions, an advent outlining the foundations in the back of the approach, lists of the mandatory apparatus and reagents, and pointers on troubleshooting and warding off recognized pitfalls.

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Colby, T. , and Waldron, J. A. (1997) Cigarette smoking: a risk factor for idiopathic pulmonary fibrosis. Am. J. Respir. Crit. Care Med. 155, 242–248. 10. , and Britton, J. (1996) Occupational exposure to metal or wood dust and aetiology of cryptogenic fibrosing alveolitis. Lancet 347, 284–289. 11. , and Kudoh, S. (2003) Diabetes mellitus may increase risk for idiopathic pulmonary fibrosis. Chest 123, 2007–2011. 12. King, T. , Tooze, J. , Schwarz, M. , Brown, K. , and Cherniack, R. M. (2001) Predicting survival in idiopathic pulmonary fibrosis: scoring system and survival model.

Gross, T. J. and Hunninghake, G. W. (2001) Idiopathic pulmonary fibrosis. N. Engl. J. Med. 345, 517–525. Pulmonary Fibrosis 31 6. , and Britton, J. (1990) What causes cryptogenic fibrosing alveolitis? A case-control study of environmental exposure to dust. BMJ 301, 1015–1017. 7. Mannino, D. , Etzel, R. , and Parrish, R. G. (1996) Pulmonary fibrosis deaths in the United States, 1979–1991. An analysis of multiple-cause mortality data. Am. J. Respir. Crit. Care Med. 153, 1548–1552. 8. , and Logan, R.

Large retrospective studies of patients with IPF showed no survival benefit with CS (12; 15,123,128). In one retrospective study from England, survival was worse among IPF patients treated with CS or CP, although this likely reflects a selection bias (15). Given the potential severe toxicities associated with CS (105,124), recent international consensus statements argue that high-dose CS should not be used to treat IPF (2,61). However, because anecdotal responses to CS are occasionally noted in patients with IPF/UIP (14), these statements acknowledge that selected patients with clinical or physiological impairment or worsening PFTs should be treated (2,61).

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